STK3 Suppresses Ovarian Cancer Progression by Activating NF-κB Signaling to Recruit CD8⁺ T-Cells

Figure 2

Overexpressed STK3 can inhibit the invasion and proliferation of ovarian cancer cells in vitro and in vivo. (a) The STK3 expression in five different cell lines measured by qRT-PCR and Western blotting. (b) The STK3 overexpression was confirmed by Western blotting and qRT-PCR in OVCAR3 and OVCAR8 cell lines. (c) Effect of the STK3 overexpression on the proliferation and colony formation of OVCAR3 and OVCAR8 cells in vitro (two-tailed Student’s -test, ; ). (d) The overexpression of STK3 arrested ovarian cancer cells at G2/M. The cell cycle distribution of OVCAR3 and OVCAR8 cells detected by flow cytometry (two- tailed Student’s -test, ). (e) The overexpression of STK3 promotes ovarian cancer cell apoptosis, as detected by cell apoptosis assays (two- tailed Student’s -test, ; ). (f) Morphologic characteristics of tumors from mice inoculated with vector and lenti-STK3/OVCAR8 cells. Tumor weights and volumes of the vector and lenti-STK3 groups are shown (two- tailed Student’s -test, ). The overexpression of STK3 can inhibit the invasion and migration of ovarian cancer cells. (g) Effect of the STK3 overexpression on invasion and migration of OVCAR3 and OVCAR8 cells by cell migration and invasion assays (two- tailed Student’s -test, ; ). Scale bar: 100 μm. (h) Effect of the STK3 overexpression on the migration of OVCAR3 and OVCAR8 cells detected by wound healing assays (two- tailed Student’s -test, ; ). Scale bar: 100 μm.

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