Immunotherapy with IgY Antibodies toward Outer Membrane Protein F Protects Burned Mice against Pseudomonas aeruginosa Infection

Figure 1

SDS-PAGE for detecting expressed and purified r-OprF. Lane M: low molecular weight protein size markers; Lane 1: precolumn lysate, Lane 2: flow through the matrix; Lane 3: washing with 20 mM imidazole; Lane 4: elution with 250 mM imidazole; Lane 5: purified r-OprF after dialysis (a). Western blotting results. Lane 1: r-OprF detected by monoclonal anti-His tag antibody; Lane M: low molecular weight protein size markers (b).

(a)

(b)