Immunotherapy with IgY Antibodies toward Outer Membrane Protein F Protects Burned Mice against Pseudomonas aeruginosa Infection

Figure 2

Precipitation and reactivity of anti-OprF IgY antibodies. SDS-PAGE of IgY precipitated with NaCl under acidic conditions. Lane 1: egg yolk; Lane 2: filtered diluted egg yolk, Lane 3: add NaCl; Lane 4: adjust pH 4; Lane 5: precipitated at room temperature for 2 h; Lane 6: purified IgY; Lane M: protein marker (a). R-OprF induced specific IgY binding to P. aeruginosa target antigen. IgY immunoreacted with r-OprF (∼48) protein (b). An indirect ELISA was used to determine the reactivity of IgY antibodies against r-OprF with P. aeruginosa strain PAO1 (c) and r-OprF (d). C-IgY served as negative controls. Values represent the (SD).

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