Tissue-Resident Type 2 Innate Lymphoid Cells Arrest Alveolarization in Bronchopulmonary Dysplasia

Figure 4

Depletion of ILC2s attenuates BPD. (a) C57BL/6 BPD mice were treated with mouse anti-CD90.2 antibody or IgG2b every alternate day from day 7 day after birth. Neonatal mice were euthanized for lungs on P14. (b) Representative hematoxylin-eosin-stained sections of the lung from C57BL/6 mice treated with saline, LPS, LPS+vehicle (IgG), and LPS+anti-CD90.2. Original magnification, ×200. (c) Representative results of ILC2s detected by flow cytometry on different postnatal days in saline, LPS+vehicle, and LPS+anti-CD90.2 groups. (d) Percentage of ILC2 in the lung on different postnatal days in saline, LPS+vehicle, and LPS+anti-CD90.2 groups. (e, f) Relative expression of IL-4 and IL-13 in the lung in saline, LPS+vehicle, and LPS+anti-CD90.2 groups, respectively. (g, h) Representative results of M1 and M2 macrophages detected by flow cytometry in saline, LPS+vehicle and LPS+anti-CD90.2, groups, respectively. (i, j) Percentage of M1 and M2 macrophages in lung in saline, LPS+vehicle, and LPS+anti-CD90.2 groups, respectively. Data are represented as . . indicates .

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