Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples
Development of Aβ1-42 detection. (a) Indirect ELISA calibration curves for Aβ1-42 using 1 : 1,000 (v/v) dilution of alpaca antiserum. (b) Optimization of coating antigen using indirect competitive ELISA. The percent binding showed a competition percentage of 6 concentrations of competitive Aβ1-42 (62, 125, 250, 500, 1,000, and 2,000 ng/ml) to a 1 : 1,000 (v/v) dilution of alpaca antiserum at 3 concentrations of coating antigen (,, and ng/ml of Aβ1-42 in coating buffer). (c) ic-competitive ELISA calibration curves for Aβ1-42 using a 1 : 1000 (v/v) dilution of alpaca antiserum. The calibration curve was obtained using analyte standards prepared in PBS buffer. (d) ic-competitive ELISA calibration curves obtained by OD.
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