Development of an Immunoassay for the Detection of Amyloid Beta 1-42 and Its Application in Urine Samples

Table 1

Results of the ELISA checker-board titration test of the binding of 6 concentrations of Aβ1-42 and 6 serially diluted antiserums by the indirect ELISA method.

Antiserum (1 : X)	OD at 450 nm
	Concentration of coated Aβ1-42 (ng/ml)
	4000	2000	1000	500	250	125
1,000	1.053	0.938	0.852	0.74	0.644	0.525
2,000	0.929	0.799	0.686	0.56	0.443	0.368
4,000	0.821	0.622	0.475	0.379	0.272	0.217
8,000	0.659	0.441	0.342	0.235	0.178	0.146
16,000	0.456	0.278	0.203	0.149	0.113	0.099
32,000	0.293	0.171	0.13	0.098	0.081	0.073