Igg-Dependent Hydrolysis of Myelin Basic Protein of Patients with Different Courses of Schizophrenia

Figure 3

(a) Gel filtration of IgG on a Superdex 200 column in acidic buffer (pH 2.6) after preincubation of the Abs in the same buffer: (—). absorbance at 280 nm (A280) that reflects the content of IgG; (■). Relative activity (RA. %) of IgGs in the hydrolysis MBP. The complete hydrolysis of MBP for 5 h was taken for 100%. (b) Electrophoretic analysis in gradient PAGE (4.5–15%) of the fraction corresponding to the central part of the peak (Figure 2(a)) before (line 1) and after (line 2) incubation with a reducing agent (10 mM DTT). Line 3—protein molecular mass markers.

(a)

(b)