Identification and Functional Analysis of EPOR⁺ Tumor-Associated Macrophages in Human Osteosarcoma Lung Metastasis

Figure 2

EPO injection led to increased EPOR⁺ lung tissue-resident macrophage infiltration by increasing proliferation. (a) Representative flow cytometry analysis and quantitative analyses GFP (EPOR) expression on F4/80⁺BrdU⁺ macrophages in the lung. . (b) Representative flow cytometry analysis of EPOR-eGFP percentage in lung F4/80⁺ macrophages after EPO injection, PBS injection as control, . (c) Quantitative analyses of F4/80⁺EPOR⁺ macrophages in lung after EPO injection, . (d) Representative flow cytometry analysis of BrdU expression in lung F4/80⁺EPOR-eGFP⁺ macrophages after EPO injection, . Dotted line: FMO (e) quantitative analyses of mean BrdU expression in lung F4/80⁺EPOR-eGFP⁺ macrophages after EPO injection, .

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