Generation of Chicken IgY against SARS-COV-2 Spike Protein and Epitope Mapping

Figure 2

IgY-S epitope mapping against the SARS-CoV-2 Proteome Microarray. Peptide microarrays were performed with IgY-S at 1 μg/ml and 10 μg/ml, and stained microarrays were scanned, and signals were collected. Fluorescence intensities of peptide spots were generated with the PepSlide Analyzer software and plotted against sequential peptides covering SARS-CoV-2 S protein from N- to C-terminus. Fluorescence intensity peaks of peptides containing the consensus motifs LDPLSET, SIIAYTMSL, QIYKTPP, AIHADQL, DLGDISGIN, and EIL are indicated. Weak signal peaks from peptide spots not of “epitope signal pattern” are also shown.