Dendritic Cells Transfected with MHC Antigenic Determinants of CBA Mice Induce Antigen-Specific Tolerance in C57Bl/6 Mice

Figure 4

Suppression of proliferation of C57BL/6 mouse splenocytes cocultured with transfected DCs through adding (a) CBA mouse splenocytes ( per group) or (b) BALB/c mouse splenocytes ( per group). Brackets indicate significant differences. (a) The data are presented as median and interquartile range (the Kruskal-Wallis test with Dunn’s corrected multiple comparisons, ). (b) The data are presented as mean and SD (one-way ANOVA with Tukey’s corrected multiple comparisons, , ). Non-EP DCs: C57Bl/6 splenocytes cocultured with nonelectroporated DCs stimulated with CBA or BALB/c splenocytes; DCp5: C57Bl/6 splenocytes cocultured with DCs electroporated with p5 stimulated with CBA or BALB/c splenocytes; DCpIL-10: C57Bl/6 splenocytes cocultured with DCs electroporated with pIL-10 stimulated with CBA or BALB/c splenocytes; DCpMHC: C57Bl/6 splenocytes cocultured with DCs electroporated with pMHC stimulated with CBA or BALB/c splenocytes; DCpMHC⁺IL-10: C57Bl/6 splenocytes cocultured with DCs electroporated with pMHC and pIL-10 stimulated with CBA (a) or BALB/c (b) splenocytes.

(a)

(b)