Dendritic Cells Transfected with MHC Antigenic Determinants of CBA Mice Induce Antigen-Specific Tolerance in C57Bl/6 Mice

Figure 5

Induction of GVHD in CBF1 (F1: C57BL/6×CBA) mice by splenocytes cocultured with the transfected DCs. (a) A GVHD induction scheme. Spleen weights were measured (b) 2 weeks or (d) 3 weeks after induction of GVHD ( per group; the Kruskal-Wallis test with Dunn’s corrected multiple comparisons, ). Frequencies of FoxP3⁺ Tregs were measured in mouse spleens (c) 2 weeks or (e) 3 weeks after induction of GVHD ( per group; one-way ANOVA with Tukey’s corrected multiple comparisons, ). Median and interquartile range. Brackets indicate significant differences, , , . Saline: administration of saline; Spl: administration of C57BL/6 mouse splenocytes; DCp5: administration of cocultures of autosplenocytes and DCs electroporated with a control plasmid p5; DCpIL-10: administration of cocultures of autosplenocytes and DCs electroporated with a pIL-10; DCpMHC: administration of cocultures of autosplenocytes and DCs electroporated with a pMHC; DCpMHC⁺IL-10: administration of cocultures of autosplenocytes and DCs electroporated with pMHC and pIL-10 simultaneously.

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