Human Adenovirus Serotype 3 Infection Modulates the Biogenesis and Composition of Lung Cell-Derived Extracellular Vesicles

Figure 5

The effect of HAdV3 infection on isolated EV classical markers. Quantification of dot blot analysis showing expression of CD9, CD63, TSG101, and GAPDH expression after 48 h and 72 h infection. EVs were probed for the presence of classical markers. (a) (i) Graph showing quantification of CD9 level after 48 h and (ii) 72 h of infection. (b) (i) CD63 after 48 h and (ii) 72 h of infection .(c) (i) TSG101 after 48 h and (ii) 72 h of infection. (d) (i) GAPDH after 48 h and (ii) 72 h of infection. Dots shown in the figure are representative of four independent experiments. Data show mean ± SEM from three independent experiments. Data show the from four independent experiments. Data shows the from four independent experiments and performed using one-way analysis of variance (ANOVA) with Tukey post hoc analysis. Statistical significance is indicated by the as follows: , , , and .

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