circ_0001588 Induces the Malignant Progression of Hepatocellular Carcinoma by Modulating miR-874/CDK4 Signaling

Figure 4

miR-874 was targeted by circ_0001588. (a, b) MicroRNAs regulated by circ_0001588. HCC cells were infected with circ_0001588-OE. (c, d) After lysis, the collected cells were incubated with A/G beads containing Ago2 or the IgG antibody. Levels of miR-874 and circ_0001588 were detected by qRT-PCR. (e) The putative binding sites between miR-874 and circ_0001588 and the mutant sites in circ_0001588-MUT reporter were displayed. (f, g) Luciferase activity was evaluated in HCC cells cotransfected with circ_0001588-MUT or circ_0001588-WT reporter and miR-874 inhibitors or mimics. ; .

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