Circular RNA ITCH Is a Tumor Suppressor in Clear Cell Renal Cell Carcinoma Metastasis through miR-106b-5p/PDCD4 Axis

Figure 3

PDCD4 mediates miR-106b-5p-dependent ccRCC cell migration and invasion. (a) The predicted binding sites between miR-106b-5p and PDCD4. (b) The dual luciferase reporter assay detects the luciferase activity of PDCD4-WT/PDCD4-MUT in OSRC-2 and SW839 cells cotransfected with miR-106b-5p mimics. (c) The relation of the miR-106b-5p expressions and PDCD4 expressions in renal cancer. (d) Western blot determines the PDCD4 expressions in OSRC-2 and SW839 cells transfected with miR-106b-5p mimics and miR-106b-5p inhibitor. (e, f) Transwell assay detects migration and invasion abilities OSRC-2 and SW839 cells transfected with NC inhibitor, miR-106b-5p inhibitor, si-PDCD4, and miR-106b-5p inhibitor. (g) Wound healing detects migration abilities of OSRC-2 and SW839 cells transfected with NC mimic, miR-106b-5p mimic, OE-PDCD4, and miR-106b-5p mimic. (h) Western blot analyzes the expressions of E-cadherin, N-cadherin in OSRC-2, SW839 cells transfected with NC inhibitor, miR-106b-5p inhibitor, si-PDCD4, and miR-106b-5p inhibitor.

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