Fel d1 Blocking Antibodies: A Novel Method to Reduce IgE-Mediated Allergy to Cats

Figure 1

Chimeric ELISA evaluation of a rabbit polyclonal anti-Fel d1 antibody (indoor) and rabbit monoclonal anti-Fel d1 (FGI) on IgE binding in different plasma samples. Chimeric ELISA analysis of IgE blocking effect of rabbit anti-Fel d1 polyclonal “Indoor Poly” (Indoor Biotechnologies, Cat# PA-FD1) and rabbit anti-Fel d1 monospecific “FGI” antibodies (FabGennix International, Cat# FELD1-121AP). Both antibodies were tested at serial dilutions of 1 : 20 to 1 : 2,000,000. Cat saliva samples incubated with buffer only (none) showed high Fel d1-to-IgE binding in cat allergic plasma, but not in noncat allergic plasma and nonallergic plasma. Binding of Fel d1 in cat allergic human plasma was reduced by Indoor Poly versus control (none) at dilutions to 1 : 2000 () but was not affected by the FGI antibody (). With noncat allergic and nonallergic plasma, Fel d1 capture was unaffected by all antibodies. Significantly different from control ().