Third-Generation Anti-CD47-Specific CAR-T Cells Effectively Kill Cancer Cells and Reduce the Genes Expression in Lung Cancer Cell Metastasis

Table 1

Primer use in qPCR.

Gene target	Primer name	Oligonucleotide sequence	PCR product size (bp)	Ref
CAR gene	TestCarF	5-TTTTACCCCT CCGACATCGC-3	253
	TestCarR	5-CAGCACGATCAGAGCCATCTTG-3
Human beta actin gene	actinF	5-ATGAAGTGTGACGTGGACAT-3	150	[21, 22]
	actinR	5-CAGGAGGAGCAATGATCTTGAT-3
Cyclooxygenase 2 gene	COX2F	5-CAAATGAGATTGTGGGAAAATTGCT-3	300	[23]
	COX2R	5-GATCATCTCTGCCTGAGTATCTT-3
Calreticulin gene	CALRF	5-AGTTCCGGCAAGTTCTACGG-3	260	[24]
	CALRR	5-CCACAGATGTCGGACCAAA-3
Glyceraldehyde 3-phosphate dehydrogenase	GAPDHF	5-TTGTCTCACTTGTTCTCT-3	87	[25]
	GAPDHR	5-ATGGGAGTTGTTTTCTTG-3