Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Figure 6

Titer of polyclonal antibodies generated against rNTHiENO. Titration by western blot of the antibodies generated against the recombinant protein. rNTHiENO was migrated on SDS-polyacrylamide gels, and after that, the membranes were electrotransferred to nitrocellulose membranes; as a first antibody, the polyclonal antibody generated at different dilutions was used; as a secondary antibody, a goat anti-rabbit alkaline phosphatase-labeled IgG was used. The image is representative of at least three independent experiments. Lane 1: preimmune serum. Lane 2: dilution 1 : 1000. Lane 3: dilution 1 : 10000. Lane 4: dilution 1 : 20000. Lane 5: dilution 1 : 30000. Lane 6: dilution 1 : 40000. The weight of the recombinant protein is indicated with an arrow.