Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Figure 8

Enolase is exposed in surface in H. influenzae typeable and nontypeable. Immunodetection by immunofluorescence indirect (IFI) in nonpermeabilized H. influenzae typeable and nontypeable strains was realized. Anti-rNTHiENO antibodies were used as a first antibody; anti-rabbit FITC-labeled IgG was used as a second antibody. Each image is a representative of at least three independent experiments and captured by a fluorescence microscope. (a) H. influenzae nontypeable (HiBUAP96) with anti-H. influenzae polyclonal antibodies as a positive control. (b) H. influenzae nontypeable (HiBUAP96) with preimmune sera as a negative control. (c) H. influenzae nontypeable (HiBUAP96) with anti-rNTHiENO polyclonal antibodies; the inset shows a digital amplification (300%) carried out with the ZEISS ZEN lite 2.1 program in order to appreciate the recognition of anti-NTHiENO antibodies in the HiBUAP96 membrane. (d) H. influenzae type b (HiBUAPNAN) with anti-rNTHiENO polyclonal antibodies; the inset shows a digital amplification (300%) carried out with the ZEISS ZEN lite 2.1 program in order to appreciate the recognition of anti-NTHiENO antibodies in the HiBUAPNAN membrane.

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