Cloning and Characterization of Immunological Properties of Haemophilus influenzae Enolase

Table 1

Biochemical characteristics of NTH. influenzae enolase.

Feature	H. influenzae	S. pneumoniae	H. sapiens
Mg^2+	D244	D242	S40
	E291	E291	D245
	D318	D318	E293
			D318
Catalytic sites	H159	H155	H158
	E168	E164	E167
	E209	E205	E210
	D318	E291	E293
	K343	D318	D318
	R372	K343	K343
	S373	R372	R372
	K394	S373	S373
		K394	K394
RNA binding (%)^a	58RFLGKGVLK66 (44%)	58RYGGLGTQK66	56RYMGKGVSK64
	103KSNFGA108 (50%)	103KGKLGA108	103KSKFGA108
	191HNLAKVLKSKGMSTA205 (60%)	187HALKKILKSRGLETA201	190HNLKNVIKEKYGKDATN206
	310GDRVQL315 (66.67%)	310GKKVQL315	312GIQV315
	400RSDRIAK406 (85.71%)	^400RTDRIAK^406	^400RSERLAK^406
		432LKK434	432LAK434
Enolase signature	340ILIKFNQIGSLTET 353	340ILIKVNQIGTLTET353	340LLLKVNQIGSVTES353
Plasminogen binding (%)^b	252FYNKENGMY260 (55.5%)	248FYDKERKVY256	251FFRSGKY257

^aHomology percent with respect to exRNA-binding sites in S. pneumoniae [17]. ^bHomology percent with respect to plasminogen-binding site in S. pneumoniae [29].