Leukocytes from Patients with Drug-Sensitive and Multidrug-Resistant Tuberculosis Exhibit Distinctive Profiles of Chemokine Receptor Expression and Migration Capacity

Figure 3

Differential expression of CCR2 and CCR4 in peripheral blood monocytes from MDR-TB and DS-TB patients. Representative zebra plots showing the strategy of analysis used to evaluate CCR2 and CCR4 expression in total CD14+ monocytes by flow cytometry. Peripheral blood mononuclear cells (PBMCs) from multidrug-resistant (MDR-TB) or drug-sensitive (DS-TB) patients were obtained at different time points before and after anti-TB treatment and employed for phenotypical characterization (a and c, respectively). (b) Frequency of CD14+CCR2+, CD14+CCR2+CCR4+, and CD14+CCR4+ subpopulations in MDR-TB patients (HD: , MDR-TB: basal, 1, and 3 moTBt , 8 moTBt , and 16 moTBt ). (d) Frequency of CD14+CCR2+, CD14+CCR2+CCR4+, and CD14+CCR4+ subpopulations in DS-TB patients (HD ; DS-TB: basal, and 1 moTBt , and 6 moTBt ). Bar graphs display . Differences between patient groups and HD at each time point were analyzed using multiple Student’s -test corrected for multiple comparisons using the Sidak–Bonferroni method. , , , and . moTBt: months of antituberculosis treatment.

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