Leukocytes from Patients with Drug-Sensitive and Multidrug-Resistant Tuberculosis Exhibit Distinctive Profiles of Chemokine Receptor Expression and Migration Capacity

Figure 7

Expression of CXCR1 and CXCR3 in CD8+T cells from MDR-TB and DS-TB patients. Representative zebra plots showing the strategy used to determine the expression of CXCR1 and CXCR3 CD3+CD8+ T cells. Peripheral blood mononuclear cells (PBMCs) from multidrug-resistant (MDR-TB) or drug-sensitive (DS-TB) patients were obtained at different times before and after anti-TB treatment and used for phenotypical characterization by flow cytometry (a and c, respectively). (b) Frequency of CD8+CXCR1+, CD8+CXCR1+CXCR3+, and CD8+CXCR3+ T cell subpopulations in MDR-TB patients (HD: , MDR-TB: basal, 1, and 3 moTBt , 8 moTBt , and 16 moTBt ). (d) Frequency CD8+CXCR1+, CD8+CXCR1+CXCR3+, and CD8+CXCR3+ T cell subpopulations in DS-TB patients (HD: , DS-TB: basal and 1 moTBt , and 6 moTBt ). Bar graphs show . Differences between patient groups and HD at each time point were analyzed using multiple Student’s -test corrected for multiple comparisons using the Sidak–Bonferroni method. , , , and . moTBt: months of antituberculosis treatment.

(a)

(b)

(c)

(d)