Research Article

miR-126 in Extracellular Vesicles Derived from Hepatoblastoma Cells Promotes the Tumorigenesis of Hepatoblastoma through Inducing the Differentiation of BMSCs into Cancer Stem Cells

Figure 5

EVs with downregulated miR-126 significantly inhibited the differentiation of BMSCs into cancer stem cells. BMSCs were divided into the following groups: control, BMSC+miR mimic EVs, BMSC+cancer EVs, BMSC+inhibitor NC EVs, BMSC+mimic NC EVs, and BMSC+miR inhibitor EVs. (a) BMSCs were cocultured with cancer EVs, mimic NC EVs, miR mimic EVs, inhibitor NC EVs, or miR inhibitor EVs. Then, the ratio of CD44+ CD90+ CD133+ cells in BMSCs was assessed by flow cytometry. (b) Oct4, SRY, IL-1α, IL-6, and TGF-β1 levels in BMSCs were assessed by RT-qPCR. (c) The content of IL-6 and TGF-β1 in BMSC supernatants was investigated by ELISA. (d) The protein levels of β-catenin and ERK in BMSCs were tested by western blot. β-Actin was applied for normalization. compared to the control. # compared to BMSC+cancer EVs. Φ compared to BMSC+inhibitor NC EVs.
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