MicroRNA-34a Promotes EMT and Liver Fibrosis in Primary Biliary Cholangitis by Regulating TGF-β1/smad Pathway

Figure 2

TGIF2 interacted with the TGF-β1/smad pathway. (a) Sequences of three TGIF2 siRNAs and negative control. (b) TGIF2 mRNA level after transfected by siRNAs. (c) TβR1 and TGF-β1 mRNA levels (for TβR1, TGIF2 siRNA vs. NC, ; for TGF-β1, TGIF2 siRNA vs. siRNA NC, ). Relative TβR1, TGF-β1, and p-smad2/3 protein expressions (for TβR1, TGIF2 siRNA vs. NC, ; for TGF-β1, TGIF2 siRNA vs. NC, ; and for p-smad2/3, TGIF2 siRNA vs. NC, ). (d) CK19, α-SMA, and collagen I mRNA levels between TGIF2 siRNA and NC (, , and ). Relative CK19, α-SMA, and collagen I protein expressions between TGIF2 siRNA and NC (, , and ).

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