MicroRNA-34a Promotes EMT and Liver Fibrosis in Primary Biliary Cholangitis by Regulating TGF-β1/smad Pathway

Figure 3

miR-34 expression in the serum of PBC patients and healthy controls and HIBEC proliferation in vitro. (a) miR-34a was significantly overexpressed in the sera of PBC patients (). (b) Expression of miR-34a of HIBEC transfected by miR-34a mimics, inhibitor, and controls (miR-34a mimics vs. NC, and miR-34a inhibitor vs. NC, ). (c) Relative cell number measured by using CCK8 (miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (d) CK19 mRNA level of each group (miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). Relative CK19 protein expression (miR-34a mimics vs. NC, ; miR-34a mimics vs. inhibitor, ; and miR-34a inhibitor vs. NC, ). (e) CK19, E-cadherin, ZO-1, and laminin 1 mRNA levels between miR-34a mimics and NC (, , , and ). CK19, E-cadherin, ZO-1, and laminin 1 protein expressions between miR-34a mimics and NC (, , , and ).

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