ZNF652-Induced circRHOT1 Promotes SMAD5 Expression to Modulate Tumorigenic Properties and Nature Killer Cell-Mediated Toxicity in Bladder Cancer via Targeting miR-3666

Figure 7

SMAD5 is essential for the functional role of circRHOT1 in BC. (a) Expression level of circRHOT1 in transfected cells measured by qRT-PCR. (b) Expression of SMAD5 in transfected cells detected by qRT-PCR. (c, d) Proliferation of transfected HT-1376 (c) and HTB9 (d) cells detected by CCK-8 assay. (e) Proliferation of transfected HT-1376 and HTB9 cells determined by EDU assay. (f) Migration of transfected HT-1376 and HTB9 cells detected by Transwell migration assay. (g) The killing of HT-1376 and HTB3 cells detected by 51Cr release assay. (h) Numbers of NK cells and transfected cells assessed by perforin polarization assay. (i, j) Conjugation assay showing the conjugates formed between NK cells and transfected HT-1376 (i) and HTB9 (j) cells. Experiments were conducted in triplicate. , , and .

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