Single Nucleotide Polymorphisms of IL-33 Gene Correlated with Renal Allograft Fibrosis in Kidney Transplant Recipients

Figure 3

Validation of association between target SNP gene (IL-33) and renal graft fibrosis. (a) IHC staining using anti-IL-33 antibodies demonstrated more deposition of IL-33 in renal tubules (red arrow) was found in graft fibrosis tissues of CAD than normal tissues. Scale bar: 25 μm. (b) Representative WB results showed IL-33 expression as well as EMT-related markers including FN and α-SMA in the CAD group exceeded those in the normal group, while E-cadherin expression was higher in the normal group than the CAD group. (c) Semiquantitative analysis results of relative protein abundances of FN, E-cadherin, α-SMA, and IL-33 were shown in each group. Values represented the (; ). Abbreviations: SNP: single nucleotide polymorphism; IHC: immunohistochemistry; CAD: chronic renal graft dysfunction; WB: western blot; EMT: epithelial-mesenchymal transition.

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