Transcriptome Analysis of Large to Giant Congenital Melanocytic Nevus Reveals Cell Cycle Arrest and Immune Evasion: Identifying Potential Targets for Treatment

Figure 5

Immune evasion in lgCMN. (a) Flow chart of MHC class I-mediated antigen processing and presentation. The abnormal parts are presented in blue fonts. The downregulated E3 ligase gene leads to the dysfunction of antigen processing in lgCMN, inducing immune evasion. This figure was created using BioRender. (b) Deficiency of tumor-infiltrating lymphocytes in lgCMN tissues. Immunohistochemical staining for SOX10 (labeling lgCMN cells), CD3 (labeling mature T lymphocytes), CD4 (labeling helper T lymphocytes), and CD8 (labeling cytotoxic T lymphocytes) in lgCMN tissues (; with halo phenomenon) was stained using the AEC chromogen to detect tumor-infiltrating lymphocytes. BCL2 (antiapoptosis related) and caspase-3 (apoptosis related) were stained using the AEC chromogen to investigate antiapoptotic activity. The red arrow points at a few CD8⁺ T cells far away from SOX10+ lgCMN cells.

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