Circular RNA hsa_circ_0000511 Improves Epithelial Mesenchymal Transition of Cervical Cancer by Regulating hsa-mir-296-5p/HMGA1

Figure 6

hsa_circ_0000511 promotes EMT of cervical cancer cells in vivo by downregulating hsa-miR-296-5p/HMGA1. Inhibition of hsa_circ_0000511 significantly reduced the tumor volume in vivo (a, b), and Western blot analysis (c) showed that the protein expression of HGMA1 was decreased in turn when hsa_circ_0000511 was silenced or hsa-miR-296-5p was activated (d), while the protein expression ration between E-cadherin and N-cadherin was opposite to that of HGMA1 (e). Meanwhile, the results of qRT-PCR showed that the expression of hsa_circ_0000511 was consistent with the protein expression of HGMA1 (f) but the expression of hsa-miR-296-5p (g) was opposite to that of hsa_circ_0000511. Besides, changes of average optical density of PCNA (h, i) were consistent with the expression of hsa_circ_0000511. ; the data were analyzed by one-way analysis of variance; vs. the control, and ; vs. si-circRNA, ^#, ^##, and ^###.

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