Mechanism of Nucleic Acid Sensing in Retinal Pigment Epithelium (RPE): RIG-I Mediates Type I Interferon Response in Human RPE

Figure 4

RIG-I mRNA is elevated in patients with GA. (a) The elevation in the levels of RIG-I mRNA in patients with GA relative to non-AMD controls was observed using RNAscope. Low levels of RIG-I signal (red dots) were detected in non-AMD control patients, whereas elevated signals were detected in RGC, ONL, INL, and RPE layers of patients with GA. Scale bars are indicated. , patient information is listed in Supplementary Table 1. DAPB was used as negative control (see Supplementary Figure 1D). In addition, the RIG-I signal in the macular region was analyzed by HALO, with the % area of RIG-I positive signals being shown in (b).

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