Suppression of DLBCL Progression by the E3 Ligase Trim35 Is Mediated by CLOCK Degradation and NK Cell Infiltration

Figure 1

Low expression of Trim35 is correlated with poor prognosis in DLBCL. (a) Immunohistochemical staining for Trim35 in human DLBCL tissues and normal lymph nodes. The specific Trim35 signal is shown in brown (DAB staining), and counterstaining was performed using hematoxylin (blue nuclei). Scale bar, 200 μm. (b, c) Summary for the entire cohort of DLBCL patients, as assessed by IHC. The value was calculated by the chi-square test. (d) Pfeiffer cells were infected with virus expressing Flag-Trim35 or empty vector. After being selected, the protein extracts were immunoblotted for the indicated proteins. (e) CCK-8 assays of stable Trim35-overexpressing cells and empty vector-expressing cells. (f) Growth curves of stable Trim35 overexpression group and empty vector group were counted. (g) Overall survival analysis of patients with high (red) versus low (blue) Trim35 protein expression based on IHC staining. Samples with Trim35 staining in <5% of cells were considered to have low expression, and those with staining >5% were considered to have high expression. All experiments were repeated at least 3 times. ^NS, , , and .

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