miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury
Caspase-1-dependent pyroptosis boosted in microglia of white matter injury mice. 300 μg/kg LPS was injected into mice intraperitoneally on postnatal day 8. 14 hours later, mice were anesthetized, and the brain was harvested. (a) H&E staining for brain sections; white matter was imaged. (b) MBP staining for brain sections. Microglia were isolated with a CD11b cell separation kit. (c) LDH is released by microglia after ex vivo culture for 24 hours. (d) FLICA assay staining in microglia for activated caspase-1, caspase-3, caspase-6, and caspase-8. (e) Immunoblot for microglia from control (Ctrl) and WMI mice. (f) Protein level quantification by normalizing with β-actin. (g) IL-1β release by microglia. (h) IL-18 release by microglia. (i) Dual-color staining for lba1 and caspase-1 in the white matter. Data are . Individual data points are displayed: , , and .