Research Article

miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury

Figure 2

Inactivation of caspase-1 attenuated the pathogenesis of WMI. 25 mg/kg caspase-1 inhibitor Vx-765 injected to the WMI mouse model i.p. Microglia were isolated with a CD11b cell separation kit. (a) FLICA assay staining for caspase 1 (representative dot plot). (b) FLICA assay staining for caspase-1 (statistical data). (c) Immunoblot for procaspase-1 and caspase-1 P20. (d) Relative protein level quantification by normalizing with β-actin. (e) IL-1β release. (f) IL-18 release. (g) Dual-color staining for lba1 and caspase-1 in the white matter. (h) LDH is released by microglia after ex vivo culture for 24 hours. (i) H&E staining for brain sections. (j) MBP staining for brain sections. Data are . Individual data points are displayed: , , and .