miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury

<div>Inactivation of caspase-1 attenuated the pathogenesis of WMI. 25 mg/kg caspase-1 inhibitor Vx-765 injected to the WMI mouse model i.p. Microglia were isolated with a CD11b cell separation kit. (a) FLICA assay staining for caspase 1 (representative dot plot). (b) FLICA assay staining for caspase-1 (statistical data). (c) Immunoblot for procaspase-1 and caspase-1 P20. (d) Relative protein level quantification by normalizing with <i>β</i>-actin. (e) IL-1<i>β</i> release. (f) IL-18 release. (g) Dual-color staining for lba1 and caspase-1 in the white matter. (h) LDH is released by microglia after ex vivo culture for 24 hours. (i) H&amp;E staining for brain sections. (j) MBP staining for brain sections. 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Journal of Immunology Research

fig2

Figure 2

Figure 2: miR-214-3p Deficiency Enhances Caspase-1-Dependent Pyroptosis of Microglia in White Matter Injury 