Asiaticoside Suppresses Gastric Cancer Progression and Induces Endoplasmic Reticulum Stress through the miR-635/HMGA1 Axis

Figure 4

Asiaticoside suppressed gastric cancer cell aggressive behaviors and induced endoplasmic reticulum stress by regulating HMGA1. (a) HMGA1 expression was tested using Western blot in AGS and HGC27 cells after treatment with AC and transfection with the HMGA1 overexpression plasmid. (b) The cell viability after AC treatment and HMGA1 overexpression was assessed by the CCK-8 assay. (c) The cell migration ability of AGS and HGC27 cells after treatment with AC and transfection with the HMGA1 overexpression plasmid was determined using the Transwell assay. (d) The levels of PCNA, E-cadherin, and N-cadherin in cells after AC treatment and HMGA1 overexpression were assessed using Western blot. (e) The levels of GRP78, Chop, and hnRNPA1 in AGS and HGC27 cells after treatment with AC and transfection with the HMGA1 overexpression plasmid were tested using Western blot. .

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