Research Article

An In Vitro Comparison of Costimulatory Domains in Chimeric Antigen Receptor T Cell for Breast Cancer Treatment

Figure 3

Comparison of immunophenotypes between CAR.MUC1-41BBζ and CAR.MUC1-CD28ζ. The phenotypic analyses of CAR T cells were performed on days 4, 11, and 14 after transduction. (a) Representative flow cytometry plot demonstrating the cell subset analyzed with fluorochrome-conjugated anti-CD3 and anti-CD56 on day 4 after T cell transduction. (b) Bar graph represents the percentage of CD3+ T cells ( from 8 donors). (c) Representative flow cytometry plot at 4 and 11 after transduction. (d) Mean percentage of CD8+: CD4+ ratio on day 4 and day 11 after transduction is shown. Data shown are from 6 donors. (e) Representative flow cytometry analysis of memory phenotype analyzed on days 4, 11, and 14 after transduction; CAR T cells were expanded in culture media containing IL-2 50 U/ml and stained with anti-CD45RO and anti-CD62L antibody (TEM: CD45RO+CD62L-, TCM: CD45RO+CD62L+, TN: CD45RO-CD62L+, and TE: CD45RO-CD62L-). (f) The mean percentage of naïve (TN), central memory (TCM), effector memory (TEM), and terminal effector (TE) T cells. Data represents as ().