IL6 Induces mtDNA Leakage to Affect the Immune Escape of Endometrial Carcinoma via cGAS-STING

Figure 4

IL6-treated EC cells inhibited the immune function of T cells through an EV transmission manner. (a) Western blot analysis of EV markers (CD9, TSG101, Alix, and Hsp90) in controls or IL6 treatment cells lysate and separated EVs, respectively. (b) Transmission electron microscopy of EVs derived from controls or IL6 treatment, respectively. (c) Nanoparticle tracking analysis of EVs derived from controls or IL6 treatment, respectively. (d) qRT-PCR analysis of cytosolic DNA (mtDNA) in EVs derived from controls, IL6 treatment, or IL6 and EthBr treatment, respectively. (e) The PD-L1 content in purified EV derived from controls, IL6 treatment, or IL6 and EthBr treatment was determined by ELISA, respectively. (f) T cells were treated with the purified EVs derived from controls, IL6 treatment, or IL6 and EthBr treatment, respectively. CD3+/CD8+ cells were measured by flow cytometry. (g) T cells were treated with the purified EVs derived from controls, IL6 treatment, or IL6 and EthBr treatment, respectively. CD4+/CD25+ cells were measured by flow cytometry. The data were represented as the ; or .

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