Semaphorin-3 Promotes Specific Immunotherapy Effects on Experimental Food Allergy

Figure 5

sema3A restores sensitivity to specific antigen-induced apoptosis of sensitized mast cells. (a, b) BMMCs were treated with the procedures denoted above each FCM panel. Gated FCM plots show apoptotic BMMC counts. Boxplots show summarized apoptotic BMMC counts of 6 independent experiments. Sens: sensitized; OVA: 5 μg/ml; BSA: 5 μg/ml; sema3A: 100 ng/ml; plexin A2-d: plexin A2-deficient BMMCs (generated by RNAi); control: BMMCs were treated with control RNAi reagents. (c, d) FA mice were treated with the procedures denoted above each FCM panel. Mast cells were gated first (presented in Figure S6), followed by counting apoptotic mast cells; gated plots show apoptotic mast cells; boxplots show summarized apoptotic mast cell counts of 6 mice per group. OVA: 100 μg/mouse; BSA: 100 μg/mouse; sema3A: 100 μg/mouse; plexin A2-Ab: antiplexin A2-Ab (50 μg/mouse/time, together with OVA challenge). The data of boxplots are presented as median (IQR). (ANOVA+Dunnett’s test), compared with group A. ^### (Student test), compared with group F.

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