Research Article

Ferric Ammonium Citrate Upregulates PD-L1 Expression through Generation of Reactive Oxygen Species

Figure 2

Effects of FAC-treated macrophages on CD4+ T cell polarization. C57BL/6J-derived macrophages were treated with 1 mM FAC for 16 h. After washing with media, cells were coincubated with BALB/c-originated CD4+ T cells for 5 d. The percentages of CD4+IFNγ+ Th1 cells (a) and CD4+CD25+Foxp3+ Treg cells (b) were measured by flow cytometry. indicates significant differences (, , and ) from the control group.
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(b)