Research Article

Ferric Ammonium Citrate Upregulates PD-L1 Expression through Generation of Reactive Oxygen Species

Figure 3

Effects of FAC on mitochondrial function. cells/mL BMDMs were stimulated with indicated concentrations of FAC for 16 h. (a) The OCR was measured using a Seahorse XF-96 Flux Analyzer. The scatterplot with the bar graph illustrates calculated respiratory parameters. (b) Aconitase activity was measured by ELISA, and (c) the amount of intracellular labile iron was given as the delta () of the fluorescence to initial fluorescence. (d) The amount of lipid peroxidation was estimated by the mean fluorescence intensity (MFI) of C11-BODIPY using flow cytometry. indicates significant differences (, , and ) from the control group.
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