miR-138-5p Inhibits Vascular Mimicry by Targeting the HIF-1α/VEGFA Pathway in Hepatocellular Carcinoma

Figure 4

miR-138-5p reduces tumour cell tube formation by targeting HIF-1α/VEGFA signalling. (a) The structure of miR-138-5p was predicted from miRTarBase (http://mirtarbase. http://cuhk.edu.cn/). (b) The predicted binding site of miR-138-5p in the 3-UTR of HIF-1α and the mutated 3-UTR of HIF-1α. (c) A luciferase reporter plasmid containing the wild-type HIF-1α 3-UTR or the mutant HIF-1α 3-UTR was transfected into 293 T cells alone or cotransfected with the NC or miR-138-5p mimic, and luciferase activity was measured. (d) and (e) qRT–PCR detection of HIF-1α and VEGFA mRNA expression in HCC cells treated with the miR-138-5p mimic (d) or inhibitor (e). (f) Western blot detection of HIF-1α and VEGFA protein expression in HCC cells transfected with the miR-138-5p mimic or inhibitor or with the HIF-1α inhibitor CAY10585 under normal oxygen and hypoxic conditions. (g) The HIF-1α inhibitor CAY10585 reversed the effects of the miR-138-5p inhibitor on tube formation by HCC cells. Data are shown as the . All experiments were repeated at least 3 times. Represents ; Represents ; Represents .

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