Deletion of Mettl3 at the Pro-B Stage Marginally Affects B Cell Development and Profibrogenic Activity of B Cells in Liver Fibrosis

Figure 4

Knockout of Mettl3 minimally affects B cell activation upon stimulation in vitro. B cells were isolated from the spleen of WT and Mettl3 cKO mice by using CD19 microbeads and treated with LPS (2 μg/ml), CD40L (100 ng/ml), anti-IgM (1 μg/ml), or TNFa (50 ng/ml). Cells were cultured for 2 days, then collected and subjected to flow cytometry. (a)–(h) Representative flow cytometry plots (a)–(d) and quantification (e)–(h) of indicated populations in indicated groups. (i) ELISA assay for IgG secretion of B cells treated with LPS (2 μg/ml), CD40L (100 ng/ml), and anti-IgM (1 μg/ml) for 2 days. (j) RT-qPCR analysis for indicated genes in B cells of indicated groups upon LPS stimulation (/group). Data in (e)–(h) were presented as with the indicated significance (, , ; student’s -test).

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