have generat monoclon bcell mous introduc homozyg nonfunct rag2 allel 1 lightchain transgen quasimonoclon mous which contain knockedin rearrang thus mous which call monob devoid cell contain preform heavi lightchain gene encod immunoglobulin antinp specif the monob mous allow examin immunoglobulin divers absenc process mediat recombin cell here report onli monob primari immunoglobulin repertoir monoclon also it secondari repertoir further diversifi vgene replac gene convers among 99 heavychain 41 lightchain gene from peripher cell monob mous were vgene replac when compar mous which has rag activ which vgene replac major diversifi mechan data suggest vgene replac mediat recombin other recombin system

Marilia Cascalho

Denise A. Martin

Jamie Wong

Queenie Lam

Matthias Wabl

Gillian E. Wu

A Mouse with a Monoclonal Primary lmmunoglobulin
Repertoire not Further Diversified by V-Gene
Replacement

femal reproduct onli system subject well defin period chang the final stage menstrual cycl mammal matur ovum prepar femal organ support fetal develop fertil once pregnanc occur both matern fetal site emit regulatori signal ensur embryo develop matern protect against graft versus host gvh reaction initi semiallogen fetus other have previous shown each day fetal develop mice character differ cytokin product detect onli proxim fetoplacent unit decidua uterus also matern lymphoid organ spleen well serum present studi concentr menstrual cycl preimplant stage pregnanc defin level gmcsf il10 il6 il3 murin uterus dure anoestrus proestrus oestrus second third day gestat show immunofluoresc elisa techniqu gmcsf maintain high level dure anoestrus proestus oestrus second day pregnanc while drop third day il3 level found elev dure proestrus second third day gestat il6 increas essenti dure proestrus wherea product il10 detect dure oestrus earli stage pregnanc immunoperoxidas stain frozen section uteri dure earli gestat period local gmcsf il3 product endometrium il10 endometrium second day pregnanc endometrium myometrium third day low level il6 could detect endometrium epithelium second day endometrium myometrium third day gestat the role il3 il10 lesser degre il6 fortifi embryo itself sinc cytokin were found produc blastocyst well these result demonstr exist specif distribut lymphokin within uterin tissu role which be discuss

Irene Athanassakis

Vagia Farmakiotis

Lina Papadimitriou

Uterine Cytokine Production During the Menstrual Cycle and Preimplantation Stages in Mice

have reinvestig import issu amphibian immunolog has been settl year sinc pioneer work triplett concern necess be expos organspecif antigen earli develop found syngen lens were reject frog xenopus laevi had been enucl eye remov dure earli larval life this reject did occur intact frog those enucl later larval adult life wherea splenocyt from intact frog did prolifer respons cocultur syngen len those from frog had been enucl ani larval stage even after metamorphosi prolifer intens both respons were shown thymus depend conclus demonstr frog immun system reject even syngeh lens enucl earli larval life began recogn syngen lens lymphoid prolifer after enucl even later life

Takeshi  Enomoto

Shin  Tochinai

Immune Response to &#8220;Self&#8221; Lens in Xenopus laevisEnucleated during Larval Life

interleukin12 il12 import cytokin maintain normal system defens bioregul the japanes herbal medicin shosaikoto tj9 has been administ 15 million japanes patient chronic liver diseas tj9 known signific suppress cancer develop liver has macrobiot effect present studi examin vitro product il12 circul mononuclear cell from liver cirrhosi patient effect tj9 il12 productionth monocyt macrophag fraction lymphocyt fraction peripher blood were obtain from 11 hcvposit liver cirrhosi patient 12 healthi subject interleukin12 level supernat were measur use elisa kit the level il12 produc patient fraction were signific lower than those produc healthi subject 001 005 howev when tj9 ad cultur il12 product level both cell fraction increas approxim three fold level from monocyt macrophag fraction were almost same those from healthi subject this effect tj9 attribut two it seven herb compon scutellaria root glycyrrhiza root one possibl mechan macrobiot effect tj9 liver cirrhosi patient may improv il12 product

Masayoshi  Yamashiki

Akira  Nishimura

Xian-Xi  Huang

Tsutomu  Nobori

Seigo  Sakaguchi

Hiroyuki  Suzuki

Effects of the Japanese Herbal Medicine &#8220;Sho-saiko-to&#8221;(TJ-9) on Interleukin-12 Production in Patients withHCV-Positive Liver Cirrhosis

have previous report readingfram usag function diversif development regul virtual all tcrb mrna transcript use singl read frame 8 week gestat age taper 50 adult life use polymeras chain reaction creat genom librari rearrang tcrb locus from thymus 77 10 16 week gestat age from adult thymus clone were random pick sequenc determin junction sequenc readingfram util the result data address hypothesi cell bear genom joint read frame one preferenti select dure fetal life this hypothesi predict read frame bias would also observ among genom joint instead observ random util three possibl dregion read frame among genom d1s1 j1s1 joint dure fetal life similar result were obtain 77 week gestat age second thymus which both rna dna were simultan isol use creat librari tcrbdj transcript rearrang conclud readingfram util random among genom d1s1jb1s1 rearrang preferenti usag singl read frame among mrna transcript tcrb transcript result preferenti transcript genom tcrb joint singl read frame tcrb transcript have longer halflif than transcript read frame two three

James F. George

Yasushi  Matsuura

Jacquelyn A. Byrne

Eugene L. Liu

Denise R. Shaw

John F. Kearney

A Developmental Bias in Reading Frame Usage by HumanFetal Thymic TCRBDJ Transcripts is not Present inGenomic TCRBDJ Rearrangements

dure embryogenesi mous thymus seed wave hematopoiet stem cell provid first peripher lymphocyt after birth known embryo thymocyt adult thymocyt have differ phenotyp function featur the identif gene express thymus onli dure embryogenesi would help understand molecular basi under characterist use mrna differenti display techniqu compar gene express between thymus kidney from embryo 1712 day adult mice this techniqu method choic compar gene express becaus abl display rapid simultan mrna complement from sever differ type cell the major drawback method lead clone mani fals posit therefor need high throughput method screen truli differenti express cdnas combin advantag from previous describ method order develop new version mrna differenti display techniqu fast cheap reliabl instead oligo prime use random hexamer revers transcript total rna 10mer primer amplif intern part cdnas obtain reproduc clean pattern discret band were abl easili identifi dnas differenti amplifi between embryo adult tissu embryo specif 5873 between thymus kidney thymus specif thi 5254 between embryo adult thymus embryo thymus specif thi 5873 cdna fragment after reamplif clone sequenc dna fragment appear most case one band correspond singl dna sequenc northern blot each candid gene recogn transcript differenti express expect thus report optim reproduc fast mrna differenti display method overcom usual problem met origin describ techniqu it report modif

Steve Pascolo

Debbie Tsoukatou

Clio Mamalaki

Identification of Thymus Specific and Developmentally
Regulated Genes by an Improved Version of the mRNA
Differential Display Technique

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Journal of Immunology Research provides a platform for scientists and clinicians working in different and diverse areas of immunology and therapy.

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Chief Editor, Professor Holland, has a background focusing on researching the development of conjunctival fibrosis and the characterisation of immune responses to potential C. trachomatis vaccine candidates.

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Journal of Immunology Research

Table of Contents

Table of Contents: 1999

A Mouse with a Monoclonal Primary lmmunoglobulin Repertoire not Further Diversified by V-Gene Replacement

Uterine Cytokine Production During the Menstrual Cycle and Preimplantation Stages in Mice

Immune Response to “Self” Lens in Xenopus laevis Enucleated during Larval Life

Effects of the Japanese Herbal Medicine “Sho-saiko-to” (TJ-9) on Interleukin-12 Production in Patients with HCV-Positive Liver Cirrhosis

A Developmental Bias in Reading Frame Usage by Human Fetal Thymic TCRBDJ Transcripts is not Present in Genomic TCRBDJ Rearrangements

Identification of Thymus Specific and Developmentally Regulated Genes by an Improved Version of the mRNA Differential Display Technique