Lipid Bilayer Composition Affects Transmembrane Protein Orientation and Function

Table 1

Outline of treatments included in the sidedness assay. One batch of proteoliposomes was prepared and split into 6 × 400 μl aliquots, each aliquot preincubated (2 minutes, room temperature) with reagents indicated, then 0 or 3 mM ouabain added, and samples incubated with ATP (60 minutes; 37°C). Adapted from [33].

Treatment		no K^+		K^+		Opened
		B	D	A	C	F	G
Pre incubation	K^+	0	0	yes	yes	yes	yes
	Ionophores	0	0	yes	yes	yes	yes
	Detergent	0	0	0	0	yes	yes
Post incubation	Ouabain	0	yes	0	yes	0	yes