The Interaction of Plasma Sialylated and Desialylated Lipoproteins with Collagen from the Intima and Media of Uninvolved and Atherosclerotic Human Aorta

Table 5

Effect of neuraminidase treatment on lipoprotein sialic acid content and lipoprotein binding to collagen.

Lipoprotein	Sialic acid content, % of control	Binding to collagen from the PG-rich layer of normal intima, % of control	Binding to collagen from the PG-rich layer of fibroatheroma, % of control
VLDL	40 ± 3*	107 ± 6	178 ± 11*
IDL	28 ± 3*	117 ± 9	214 ± 12*
LDL	27 ± 2*	108 ± 10	347 ± 21*
HDL	36 ± 3*	114 ± 8	117 ± 9

Sialylated lipoproteins were treated with 0.02 U/mL neuraminidase from Arthrobacter ureafaciens at 37°C for 6 h. Control lipoproteins were incubated in the absence of neuraminidase. Lipoproteins were then reisolated by ultracentrifugation, and microtiter well binding assay was performed as described in Section 2. Values are the means ± S.E.M (n = 4); *significant difference from control (𝑃<0.05).