Lipid Rafts: Keys to Sperm Maturation, Fertilization, and Early Embryogenesis
Figure 3
Distribution and function of CD9 and CD81 in murine oocyte. (a) The distribution of CD9 (green) is distinct from that of CD81 (red). CD9 localizes over the entire surface membrane, except for the MII plate, whereas CD81 shows patches with a low frequency. Scale bar = 10 μm. (b) Cell surface-bound sperm shows colocalization of CD9 and CD81 with sperm nuclei (blue). Scale bar = 10 μm. (c) Pretreatment of an oocyte with cyclodextrin (CD) prevents sperm from fusing and fertilizing the oocyte. The same phenomenon is observed in the treatment of filipin.
