Human Plasma Very Low-Density Lipoproteins Are Stabilized by Electrostatic Interactions and Destabilized by Acidic pH
Change from mildly basic to mildly acidic conditions does not affect the protein conformation or the net change on VLDL. VLDL solutions in 10 mM Na phosphate buffer at pH 6.2 or 7.8 were analyzed by far-UV CD spectroscopy for the apolipoprotein secondary structure (a) or by agarose gel electrophoresis for the net charge on the lipoprotein (b).
Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.