LysoPC and PAF Trigger Arachidonic Acid Release by Divergent Signaling Mechanisms in Monocytes

Figure 5

cPLA2 activity is differentially regulated by lysoPC and PAF. (a) cPLA₂ enzyme assays were done on cell lysates treated with lysoPC or PAF. (b) and (c) cPLA₂ activity was determined in lysates from THP-1 monocytes treated with different chemical inhibitors before stimulation with (b) lysoPC or (c) PAF. Data are expressed as means ± SD of duplicate determinations within separate experiments. Data shown are one representative of three independent experiments.

(a)

(b)

(c)