The F-actin distribution in RBL-2H3 and D1 cells reflects their morphology. Actin filaments in RBL-2H3 cells lie under the plasma membrane following the spindle shape of the cells and in association with microvilli. The actin cytoskeleton is altered in D1 cells and the actin filaments are concentrated in large membrane ruffles. The cells were fixed, permeabilized, and stained with phalloidin conjugated to Alexa 488. Samples were examined using a Leica TCS-NT laser scanning confocal microscope.
