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Journal of Lipids
Volume 2012, Article ID 236807, 8 pages
http://dx.doi.org/10.1155/2012/236807
Research Article

Use of the Signature Fatty Acid 16:1ω5 as a Tool to Determine the Distribution of Arbuscular Mycorrhizal Fungi in Soil

1Institute of Biology, Ecology Group, Humboldt-Universität zu Berlin, Philippstraße 13, 10115 Berlin, Germany
2Department of Natural Resource Sciences, MacCampus, McGill University, 21,111 Lakeshore Road, Ste Anne de Bellevue, QC, Canada H9X 3V9
3Institute of Vegetable and Ornamental Crops Großbeeren, Theodor-Echtermeyer-Weg 1, 14979 Großbeeren, Germany
4Faculty of Food and Agriculture, UAE University, Jimi 1 Campus, Building 52, P.O. Box 17555, Al Ain, Abu Dhabi, UAE

Received 28 March 2012; Accepted 14 May 2012

Academic Editor: Paul R. Herron

Copyright © 2012 Christopher Ngosong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. Lechevalier and M. P. Lechevalier, “Chemotaxonomic use of lipids-an overview,” in Microbial Lipids, C. Ratledge and S. G. Wilkinson, Eds., pp. 869–902, Academic Press, London, UK, 1988. View at Google Scholar
  2. A. Tunlid and D. C. White, “Use of lipid biomarkers in environmental samples,” in Analytical Microbiology Methods, Chromatography and Mass Spectrometry, A. Fox, S. L. Morgan, L. Larsson, and G. Odham, Eds., Plenum Press, New York, NY, USA, 1990. View at Google Scholar
  3. R. E. Koske and J. N. Gemma, “A modified procedure for staining roots to detect VA mycorrhizas,” Mycological Research, vol. 92, pp. 486–505, 1989. View at Google Scholar
  4. D. C. White, J. O. Stair, and D. B. Ringelberg, “Quantitative comparisons of in situ microbial biodiversity by signature biomarker analysis,” Journal of Industrial Microbiology, vol. 17, no. 3-4, pp. 185–196, 1996. View at Google Scholar · View at Scopus
  5. L. Zelles, “Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review,” Biology and Fertility of Soils, vol. 29, no. 2, pp. 111–129, 1999. View at Publisher · View at Google Scholar · View at Scopus
  6. R. G. Joergensen and F. Wichern, “Quantitative assessment of the fungal contribution to microbial tissue in soil,” Soil Biology and Biochemistry, vol. 40, no. 12, pp. 2977–2991, 2008. View at Publisher · View at Google Scholar · View at Scopus
  7. L. Ruess and P. M. Chamberlain, “The fat that matters: soil food web analysis using fatty acids and their carbon stable isotope signature,” Soil Biology and Biochemistry, vol. 42, no. 11, pp. 1898–1910, 2010. View at Publisher · View at Google Scholar · View at Scopus
  8. S. E. Smith and D. J. Read, Mycorrhizal Symbioses, Academic Press, London, UK, 3rd edition, 2008.
  9. S. D. Veresoglou, G. Menexes, and M. C. Rillig, “Do arbuscular mycorrhizal fungi affect the allometric partition of host plant biomass to shoots and roots? A meta-analysis of studies from 1990 to 2010,” Mycorrhiza, vol. 22, pp. 227–235, 2011. View at Publisher · View at Google Scholar · View at Scopus
  10. E. Neumann and E. George, “Colonisation with the arbuscular mycorrhizal fungus Glomus mosseae (Nicol. & Gerd.) enhanced phosphorus uptake from dry soil in Sorghum bicolor (L.),” Plant and Soil, vol. 261, no. 1-2, pp. 245–255, 2004. View at Publisher · View at Google Scholar · View at Scopus
  11. M. Govindarajulu, P. E. Pfeffer, H. Jin et al., “Nitrogen transfer in the arbuscular mycorrhizal symbiosis,” Nature, vol. 435, no. 7043, pp. 819–823, 2005. View at Publisher · View at Google Scholar · View at Scopus
  12. Y. Tanaka and K. Yano, “Nitrogen delivery to maize via mycorrhizal hyphae depends on the form of N supplied,” Plant, Cell and Environment, vol. 28, no. 10, pp. 1247–1254, 2005. View at Publisher · View at Google Scholar · View at Scopus
  13. M. H. Ryan, G. A. Chilvers, and D. C. Dumaresq, “Colonisation of wheat by VA-mycorrhizal fungi was found to be higher on a farm managed in an organic manner than on a conventional neighbour,” Plant and Soil, vol. 160, no. 1, pp. 33–40, 1994. View at Google Scholar · View at Scopus
  14. M. E. Gavito and M. H. Miller, “Changes in mycorrhiza development in maize indeed by crop management practices,” Plant and Soil, vol. 198, no. 2, pp. 185–192, 1998. View at Publisher · View at Google Scholar · View at Scopus
  15. F. Oehl, E. Sieverding, P. Mäder et al., “Impact of long-term conventional and organic farming on the diversity of arbuscular mycorrhizal fungi,” Oecologia, vol. 138, no. 4, pp. 574–583, 2004. View at Publisher · View at Google Scholar · View at Scopus
  16. L. K. Abbott, A. D. Robson, and G. De Boer, “The effects of phosphorus on the formation of hyphae in soil by the vesicular arbuscular mycorrhizal fungus Glomus fasiculatum,” New Phytologist, vol. 97, pp. 437–446, 1984. View at Google Scholar
  17. P. Kormanik and A. C. McGraw, “Quantification of vesicular-arbuscular mycorrhizae in plant roots,” in Methods and Principals of Mycorrhizal Research, N. C. Schenck, Ed., pp. 37–45, The American Phytopathological Society, Minn, USA, 1982. View at Google Scholar
  18. P. A. Olsson, I. M. Van Aarle, M. E. Gavito, P. Bengtson, and G. Bengtsson, “13C incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza,” Applied and Environmental Microbiology, vol. 71, no. 5, pp. 2592–2599, 2005. View at Publisher · View at Google Scholar · View at Scopus
  19. D. Redecker, “Specific PCR primers to identify arbuscular mycorrhizal fungi within colonized roots,” Mycorrhiza, vol. 10, no. 2, pp. 73–80, 2000. View at Google Scholar · View at Scopus
  20. D. Schwarzott and A. Schüßler, “A simple and reliable method for SSU rRNA gene dna extraction, amplification, and cloning from single AM fungal spores,” Mycorrhiza, vol. 10, no. 4, pp. 203–207, 2001. View at Google Scholar · View at Scopus
  21. P. A. Olsson, E. Bååth, I. Jakobsen, and B. Soderstrom, “The use of phospholipid and neutral lipid fatty acids to estimate biomass of arbuscular mycorrhizal fungi in soil,” Mycological Research, vol. 99, no. 5, pp. 623–629, 1995. View at Google Scholar · View at Scopus
  22. P. A. Olsson, R. Francis, D. J. Read, and B. Söderström, “Growth of arbuscular mycorrhizal mycelium in calcareous dune sand and its interaction with other soil microorganisms as estimated by measurement of specific fatty acids,” Plant and Soil, vol. 201, no. 1, pp. 9–16, 1998. View at Publisher · View at Google Scholar · View at Scopus
  23. P. A. Olsson, “Signature fatty acids provide tools for determination of the distribution and interactions of mycorrhizal fungi in soil,” FEMS Microbiology Ecology, vol. 29, no. 4, pp. 303–310, 1999. View at Publisher · View at Google Scholar · View at Scopus
  24. J. Larsen and L. Bødker, “Interactions between pea root-inhabiting fungi examined using signature fatty acids,” New Phytologist, vol. 149, no. 3, pp. 487–493, 2001. View at Publisher · View at Google Scholar · View at Scopus
  25. I. M. Van Aarle and P. A. Olsson, “Fungal lipid accumulation and development of mycelial structures by two arbuscular mycorrhizal fungi,” Applied and Environmental Microbiology, vol. 69, no. 11, pp. 6762–6767, 2003. View at Publisher · View at Google Scholar · View at Scopus
  26. P. A. Olsson and P. Wilhelmsson, “The growth of external AM fungal mycelium in sand dunes and in experimental systems,” Plant and Soil, vol. 226, no. 2, pp. 161–169, 2000. View at Publisher · View at Google Scholar · View at Scopus
  27. K. Hedlund, “Soil microbial community structure in relation to vegetation management on former agricultural land,” Soil Biology and Biochemistry, vol. 34, no. 9, pp. 1299–1307, 2002. View at Publisher · View at Google Scholar · View at Scopus
  28. T. C. Balser, K. K. Treseder, and M. Ekenler, “Using lipid analysis and hyphal length to quantify AM and saprotrophic fungal abundance along a soil chronosequence,” Soil Biology and Biochemistry, vol. 37, no. 3, pp. 601–604, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. C. L. Hebel, J. E. Smith, and K. Cromack, “Invasive plant species and soil microbial response to wildfire burn severity in the Cascade Range of Oregon,” Applied Soil Ecology, vol. 42, no. 2, pp. 150–159, 2009. View at Publisher · View at Google Scholar · View at Scopus
  30. Y. Huang, K. Michel, S. An, and S. Zechmeister-Boltenstern, “Changes in microbial-community structure with depth and time in a chronosequence of restored grassland soils on the Loess Plateau in north-west China,” Journal of Plant Nutrition Soil Science, vol. 174, pp. 765–774, 2011. View at Google Scholar
  31. S. Royer-Tardif, R. L. Bradley, and W. F. J. Parsons, “Evidence that plant diversity and site productivity confer stability to forest floor microbial biomass,” Soil Biology and Biochemistry, vol. 42, no. 5, pp. 813–821, 2010. View at Publisher · View at Google Scholar · View at Scopus
  32. C. B. Marshall, J. R. McLaren, and R. Turkington, “Soil microbial communities resistant to changes in plant functional group composition,” Soil Biology and Biochemistry, vol. 43, no. 1, pp. 78–85, 2011. View at Publisher · View at Google Scholar · View at Scopus
  33. K. J. van Groenigen, J. Bloem, E. Bååth et al., “Abundance, production and stabilization of microbial biomass under conventional and reduced tillage,” Soil Biology and Biochemistry, vol. 42, no. 1, pp. 48–55, 2010. View at Publisher · View at Google Scholar · View at Scopus
  34. H. Yao and F. Wu, “Soil microbial community structure in cucumber rhizosphere of different resistance cultivars to fusarium wilt,” FEMS Microbiology Ecology, vol. 72, no. 3, pp. 456–463, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. K. Bradley, R. A. Drijber, and J. Knops, “Increased N availability in grassland soils modifies their microbial communities and decreases the abundance of arbuscular mycorrhizal fungi,” Soil Biology and Biochemistry, vol. 38, no. 7, pp. 1583–1595, 2006. View at Publisher · View at Google Scholar · View at Scopus
  36. N. Aliasgharzad, L. M. Mårtensson, and P. A. Olsson, “Acidification of a sandy grassland favours bacteria and disfavours fungal saprotrophs as estimated by fatty acid profiling,” Soil Biology and Biochemistry, vol. 42, no. 7, pp. 1058–1064, 2010. View at Publisher · View at Google Scholar · View at Scopus
  37. P. A. Olsson, J. Rahm, and N. Aliasgharzad, “Carbon dynamics in mycorrhizal symbioses is linked to carbon costs and phosphorus benefits,” FEMS Microbiology Ecology, vol. 72, no. 1, pp. 125–131, 2010. View at Publisher · View at Google Scholar · View at Scopus
  38. T. K. Schnoor, L. M. Mårtensson, and P. A. Olsson, “Soil disturbance alters plant community composition and decreases mycorrhizal carbon allocation in a sandy grassland,” Oecologia, vol. 167, pp. 809–819, 2011. View at Publisher · View at Google Scholar · View at Scopus
  39. P. Nichols, B. K. Stulp, J. G. Jones, and D. C. White, “Comparison of fatty acid content and DNA homology of the filamentous gliding bacteria Vitreoscilla, Flexibacter, Filibacter,” Archives of Microbiology, vol. 146, no. 1, pp. 1–6, 1986. View at Google Scholar · View at Scopus
  40. L. Zelles, “Phospholipid fatty acid profiles in selected members of soil microbial communities,” Chemosphere, vol. 35, no. 1-2, pp. 275–294, 1997. View at Publisher · View at Google Scholar · View at Scopus
  41. B. Bago, P. E. Pfeffer, W. Zipfel, P. Lammers, and Y. Shachar-Hill, “Tracking metabolism and imaging transport in arbuscular mycorrhizal fungi,” Plant and Soil, vol. 244, no. 1-2, pp. 189–197, 2002. View at Publisher · View at Google Scholar · View at Scopus
  42. P. E. Pfeffer, D. D. Douds, G. Bécard, and Y. Shachar-Hill, “Carbon uptake and the metabolism and transport of lipids in an arbuscular mycorrhiza,” Plant Physiology, vol. 120, no. 2, pp. 587–598, 1999. View at Google Scholar · View at Scopus
  43. M. E. Gavito and P. A. Olsson, “Allocation of plant carbon to foraging and storage in arbuscular mycorrhizal fungi,” FEMS Microbiology Ecology, vol. 45, no. 2, pp. 181–187, 2003. View at Publisher · View at Google Scholar · View at Scopus
  44. P. A. Olsson and N. C. Johnson, “Tracking carbon from the atmosphere to the rhizosphere,” Ecology Letters, vol. 8, no. 12, pp. 1264–1270, 2005. View at Publisher · View at Google Scholar · View at Scopus
  45. B. Drigo, A. S. Pijl, H. Duyts et al., “Shifting carbon flow from roots into associated microbial communities in response to elevated atmospheric CO2,” Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 24, pp. 10938–10942, 2010. View at Publisher · View at Google Scholar · View at Scopus
  46. C. Ngosong, M. Jarosch, J. Raupp, E. Neumann, and L. Ruess, “The impact of farming practice on soil microorganisms and arbuscular mycorrhizal fungi: crop type versus long-term mineral and organic fertilization,” Applied Soil Ecology, vol. 46, no. 1, pp. 134–142, 2010. View at Publisher · View at Google Scholar · View at Scopus
  47. R. L. Peterson, A. E. Ashford, and W. G. Allaway, “Vesicular-arbuscular mycorrhizal associations of vascular plants on Heron Island, a Great Barrier Reef coral cay,” Australian Journal of Botany, vol. 33, no. 6, pp. 669–676, 1985. View at Google Scholar · View at Scopus
  48. D. C. Ianson and M. F. Allen, “The effect of soil texture on extraction of vesicular-arbuscular mycorrhizal spores from arid soils,” Mycologia, vol. 78, pp. 168–164, 1986. View at Google Scholar
  49. A. Thomas, D. P. Nicholas, and D. Parkinson, “Modifications of the agar film technique for assaying lengths of mycelium in soil,” Nature, vol. 205, no. 4966, p. 105, 1965. View at Publisher · View at Google Scholar · View at Scopus
  50. E. I. Newman, “A method of estimating the total length of root in a sample,” Journal of Applied Ecology, vol. 3, pp. 139–145, 1966. View at Google Scholar
  51. D. Tennant, “A test of a modified line intersect method of estimating root length,” Journal of Ecology, vol. 63, pp. 995–1001, 1975. View at Google Scholar
  52. Å. Frostegård, A. Tunlid, and E. Bååth, “Phospholipid fatty acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals,” Applied and Environmental Microbiology, vol. 59, no. 11, pp. 3605–3617, 1993. View at Google Scholar · View at Scopus
  53. C. Ngosong, J. Raupp, S. Scheu, and L. Ruess, “Low importance for a fungal based food web in arable soils under mineral and organic fertilization indicated by Collembola grazers,” Soil Biology and Biochemistry, vol. 41, no. 11, pp. 2308–2317, 2009. View at Publisher · View at Google Scholar · View at Scopus
  54. J. H. Graham, N. C. Hodge, and J. B. Morton, “Fatty acid methyl ester profiles for characterization of glomalean fungi and their endomycorrhizae,” Applied and Environmental Microbiology, vol. 61, no. 1, pp. 58–64, 1995. View at Google Scholar · View at Scopus
  55. P. A. Olsson, L. Larsson, B. Bago, H. Wallander, and I. M. Van Aarle, “Ergosterol and fatty acids for biomass estimation of mycorrhizal fungi,” New Phytologist, vol. 159, no. 1, pp. 7–10, 2003. View at Publisher · View at Google Scholar · View at Scopus
  56. StatSoft, Statistica. Version 6.0 for windows, StatSoft, Tusla, Okla, USA, 2001.
  57. R. Madan, C. Pankhurst, B. Hawke, and S. Smith, “Use of fatty acids for identification of AM fungi and estimation of the biomass of AM spores in soil,” Soil Biology and Biochemistry, vol. 34, no. 1, pp. 125–128, 2002. View at Publisher · View at Google Scholar · View at Scopus
  58. P. A. Olsson and A. Johansen, “Lipid and fatty acid composition of hyphae and spores of arbuscular mycorrhizal fungi at different growth stages,” Mycological Research, vol. 104, no. 4, pp. 429–434, 2000. View at Publisher · View at Google Scholar · View at Scopus
  59. S. P. Bentivenga and J. B. Morton, “Stability and heritability of fatty acid methyl ester profiles of glomalean endomycorrhizal fungi,” Mycological Research, vol. 98, no. 12, pp. 1419–1426, 1994. View at Google Scholar · View at Scopus
  60. K. Sakamoto, T. Iijima, and R. Higuchi, “Use of specific phospholipid fatty acids for identifying and quantifying the external hyphae of the arbuscular mycorrhizal fungus Gigaspora rosea,” Soil Biology and Biochemistry, vol. 36, no. 11, pp. 1827–1834, 2004. View at Publisher · View at Google Scholar · View at Scopus
  61. Lee Pau Ju and R. E. Koske, “Gigaspora gigantea: seasonal abundance and ageing of spores in a sand dune,” Mycological Research, vol. 98, no. 4, pp. 453–457, 1994. View at Google Scholar · View at Scopus
  62. J. P. Clapp, J. P. Young, J. W. Merryweather, and A. H. Fitter, “Diversity of fungal symbionts in arbuscular mycorrhizas from a natural community,” New Phytologist, vol. 130, no. 2, pp. 259–265, 1995. View at Google Scholar · View at Scopus
  63. P. L. Staddon, C. B. Ramsey, N. Ostle, P. Ineson, and A. H. Fitter, “Rapid turnover of hyphae of mycorrhizal fungi determined by AMS microanalysis of 14C,” Science, vol. 300, no. 5622, pp. 1138–1140, 2003. View at Publisher · View at Google Scholar · View at Scopus
  64. P. D. Steinberg and M. C. Rillig, “Differential decomposition of arbuscular mycorrhizal fungal hyphae and glomalin,” Soil Biology and Biochemistry, vol. 35, no. 1, pp. 191–194, 2003. View at Publisher · View at Google Scholar · View at Scopus