A Nonradioactive Fluorimetric SPE-Based Ceramide Kinase Assay Using NBD-C6-Ceramide
Figure 5
CERK activity in cultured cells. CERK activity towards NBD-C6-Cer was determined in lysates from CHO cells, transfected with pCMV-Tag2B (mock) or pHVO001, coding for a Flag-HsCERK fusion [8], using the fluorescence assay (black bars) or the radiometric assay (grey bars, mean ± SEM, ). CERK activity is expressed as nmol per mg protein per min (nmol/min·mg protein).