Molecular Characterization of Lipopolysaccharide Binding to Human α-1-Acid Glycoprotein
Figure 5
The protective effect of AGP against LPS-induced cytotoxicity in HEK293 cell culture. (a) Top panel. The percentage of cell growth in the presence of increasing concentrations of E. coli O127:B8 LPS. (○) 0 mg/mL AGP; (■) 0.2 mg/mL AGP; (▲) 1 mg/mL AGP. Bottom panel. The percentage of cell viability upon exposure to 100 μg/mL E. coli O127:B8 LPS, in the presence and absence of AGP. (b) Top panel. The percentage of cell growth in the presence of increasing concentrations of E. coli O111:B4 LPS. (○) 0 mg/mL AGP; (■) 0.2 mg/mL AGP; (▲) 1 mg/mL AGP. Bottom panel. The percentage of cell viability upon exposure to 100 μg/mL E. coli O111:B4 LPS, in the presence and absence of AGP. The inset table documents the IC50 values for LPS in the presence of increasing AGP levels.
